細胞名稱
SK-NEP-1人腎母細胞瘤細胞
描述
超微結構有少許微絨毛,連接復合物,形態完整的高爾基體,內質網多為光滑型,脂滴,沒有病毒棵粒。
動物種別
人
性別
女
組織來源
腎,肋膜滲出液
形態
上皮細胞
培養基和添加劑
McCOY's 5A(SIGMA,貨號M4892,添加NaHCO3 2.2g/L),85%;優質胎牛血清,15%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。
供應限制
A。僅供研究之用。
REFERENCE
Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press; 1975, pp. 115-159. Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Fogh J . C*tion, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Orkin SH , et al. Development of homozygosity for chromosome 11p markers in Wilms' tumour. Nature 309: 172-174, 1984. PubMed: 6325937 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.