細胞名稱

C6大鼠膠質瘤細胞

描述

膠質細胞株C6是由Benda等用N-nitrosomethylurea誘導的大鼠膠質瘤克隆，并經過一系列的體外培養和動物傳代交替后建成的。 當細胞從低密度生長到滿瓶時，S-100產量增加10倍。

動物種別

大鼠

性別

***

組織來源

膠質瘤，腦，膠質細胞

形態

成纖維細胞

培養基和添加劑

F12K培養基(SIGMA,貨號N3520，添加NaHCO3 2.5g/L)，82.5%；馬血清，15%；優質胎牛血清，2.5%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應限制

A。僅供研究之用。

REFERENCE

Science 161: 370-371, 1968. PubMed: 4873531 Fed. Proc. 27: 720, 1968. J. Virol. 70: 8765-8772, 1996. PubMed: 8971005 Reznikoff CA , et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800 Terzaghi M , Little JB . Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940 Mondal S , Heidelberger C . Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242 Smith GJ , et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183 Rapp UR , et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619 Reznikoff CA , et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355 Jain MK , et al. Molecular cloning and characterization of SmLIM, a developmentally regulated LIM protein preferentially expressed in aortic smooth muscle cells. J. Biol. Chem. 271: 10194-10199, 1996. PubMed: 8626582 Chinese Journal of Cancer 21(10):1106-1108, 2002 J Cancer Prev Treat 29(4):280-282,2002 Natl Med J China 82(2):90-93,2002 Jiangsu Med J. 28(S1015):343-345,2002 Chinese Journal of Cancer 22(9):959-963,2003 Research in Tumor Prev Treat 30(3):176-179,2003 Research in Tumor Prev Treat 30(5):357-358,2003 China J Cancer Prev Treat 10(5):490-492,2003 Journal of Shandong University (Health Sciences) 41(4):394-396,2003 J Fujian Med Univ 37(3):269-272,2003