專業提供Anti-Phospho-HER3 (Tyr1222)磷酸化HER3受體抗體,廣泛應用于免疫組化,ELISA,Western Blot IHC-F(免疫組化-冰凍切片),IHC-P (免疫組化-石蠟切片)IP(免疫沉淀), Flow-Cyt等方面。
免疫組化實驗注意事項: 實驗所用主要為組織標本和細胞標本兩大類,前者包括石蠟切片(病理大片和組織芯片)和冰凍切片,后者包括組織印片、細胞爬片和細胞涂片。 其中石蠟切片是制作組織標本zui常用、zui基本的方法,對于組織形態保存好,且能作連續切片,有利于各種染色對照觀察;還能*存檔,供回顧性研究;石蠟切片制作過程對組織內抗原暴露有一定的影響,但可進行抗原修復,是免疫組化中*的組織標本制作方法。 產品編號 Rs-3219R 英文名稱 Anti-Phospho-HER3 (Tyr1222) 中文名稱 磷酸化HER3受體抗體 別 名 ERBB3; c erbB 3; c erbB3; ERBB3 protein; erbB3 S; Glial growth factor receptor; HER 3; HER3; LCCS2; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; proto-oncogene-like protein c ErbB 3; proto-oncogene-like protein c ErbB3; receptor tyrosine protein kinase ERB3; Receptor tyrosine protein kinase erbB 3; Receptor tyrosine protein kinase erbB3; Tyrosine kinase type cell surface receptor HER3. 背景介紹:ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. Isoform 2 lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized. Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2); also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. 產品類型 一抗 磷酸化抗體 抗體來源 Rabbit 克隆號 Polyclonal 交叉反應 hu, rat, mo 蛋白分子量 Predicted Molecular Weight: 148kDa 性狀 Lyophilized or Liquid 濃度 1mg/1ml 免疫原 KLH conjugated Synthesised phosphopeptide derived from human HER3 around the phosphorylation site of Tyr1222 亞型 IgG 純化方法 affinity purified by Protein A 儲存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 產品應用 WB=1:100-500 Elisa=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1/20-1/100 (石蠟切片需做抗原修復) Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. 研究領域 腫瘤 免疫學 信號轉導 生長因子和激素 轉錄調節因子 激酶和磷酸酶 細胞類型標志物 保存 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.