專業提供Anti-Hes5 發狀分裂相關增強子-5抗體,廣泛應用于免疫組化,ELISA,Western Blot IHC-F(免疫組化-冰凍切片),IHC-P (免疫組化-石蠟切片)IP(免疫沉淀), Flow-Cyt等方面。
免疫組化實驗注意事項: 實驗所用主要為組織標本和細胞標本兩大類,前者包括石蠟切片(病理大片和組織芯片)和冰凍切片,后者包括組織印片、細胞爬片和細胞涂片。 其中石蠟切片是制作組織標本zui常用、zui基本的方法,對于組織形態保存好,且能作連續切片,有利于各種染色對照觀察;還能*存檔,供回顧性研究;石蠟切片制作過程對組織內抗原暴露有一定的影響,但可進行抗原修復,是免疫組化中*的組織標本制作方法。 產品編號 Rs-3767R 英文名稱 Anti-Hes5 中文名稱 發狀分裂相關增強子-5抗體 別 名 Hairy and enhancer of split 5; HES 5; HES5 protein; MGI:104876. 抗體介紹:The transcription factor Hes5 (Hairy and enhancer of split 5) is a basic helix-loop-helix (bHLH) transcriptional repressor that inhibits neural differentiation. The Hes gene products Hes1, Hes3 and Hes5, are essential for the maintenance of neural stem cells and to ensure that neuronal cells are made both in correct numbers and full diversity. Hes proteins antagonise activator bHLH proteins, such Mash1, Math and Neurogenin, which promote neuronal fate determination and induce expression of Notch ligands such as Delta. These ligands upregulate Hes1 and Hes5 gene expression in neighbouring cells, ensuring that these are maintained in an undifferentiated state. 產品類型 一抗 抗體來源 Rabbit 克隆號 Polyclonal 交叉反應 hu, rat, mo 蛋白分子量 Predicted Molecular Weight: 19kDa 性狀 Lyophilized or Liquid 濃度 1mg/1ml 免疫原 KLH conjugated synthetic peptide derived from ab abc031 亞型 IgG 純化方法 affinity purified by Protein A 儲存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 產品應用 WB=1:100-500 Elisa=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1/20-1/100 (石蠟切片需做抗原修復)Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. 研究領域 腫瘤 細胞生物 免疫學 信號轉導 轉錄調節因子 保存 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 公司提供的Anti-Hes5 發狀分裂相關增強子-5抗體,已被國內外廣大科研工作者使用,廣泛用于分子生物學、免疫學等試驗中。