Anti-Di Methyl-Histone H3 （Lys4） 二甲基組蛋白H3抗體
專業提供 Anti-Di Methyl-Histone H3 （Lys4） 二甲基組蛋白H3抗體廣泛應用于免疫組化，ELISA，Western Blot IHC-F（免疫組化－冰凍切片），IHC-P （免疫組化－石蠟切片）IP（免疫沉淀）， Flow-Cyt等方面。
產品編號 Rs-3770R
英文名稱 Anti-Di Methyl-Histone H3 （Lys4）
中文名稱 二甲基組蛋白H3抗體
別 名 H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene.
抗體介紹：Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins （H2A, H2B, H3 and H4）, is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.
產品類型 一抗 甲基化抗體
抗體來源 Rabbit
克隆號 Polyclonal
交叉反應 hu, rat, mo
蛋白分子量 Predicted Molecular Weight: 15kDa
性狀 Lyophilized or Liquid
濃度 1mg/1ml
免疫原 KLH conjugated synthetic peptide derived from hu Histone H3 （Di Methyl Lys4）.
亞型 IgG
純化方法 affinity purified by Protein A
儲存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產品應用 WB=1:100-500 Elisa=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1/20-1/100
（石蠟切片需做抗原修復）
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
研究領域 腫瘤 細胞生物 免疫學 染色質和核信號 轉錄調節因子
保存 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
公司提供的 已被國內外廣大科研工作者使用，廣泛用于分子生物學、免疫學等試驗中。
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