專業提供Anti-Acetyl-Histone H4 (Lys8)乙酰化組蛋白H4抗體,廣泛應用于免疫組化,ELISA,Western Blot IHC-F(免疫組化-冰凍切片),IHC-P (免疫組化-石蠟切片)IP(免疫沉淀), Flow-Cyt等方面。 產品編號 Rs-3747R 英文名稱 Anti-Acetyl-Histone H4 (Lys8) 中文名稱 乙?;M蛋白H4抗體 別 名 H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; methylated histone H4. 抗體介紹:Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximay 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated. 產品類型 一抗 乙?;贵w 抗體來源 Rabbit 克隆號 Polyclonal 交叉反應 hu, rat, mo 性狀 Lyophilized or Liquid 濃度 1mg/1ml 免疫原 KLH conjugated synthetic peptide derived from hu Acetyl-Histone H4 (Lys8). 亞型 IgG 純化方法 affinity purified by Protein A 儲存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 產品應用 WB=1:100-500 Elisa=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1/20-1/100 (石蠟切片需做抗原修復)Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. 研究領域 細胞生物 免疫學 染色質和核信號 轉錄調節因子 保存 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.