上海研盟生物科技有限公司Anti-TNF-alpha(1F6)抗體,腫瘤壞死因子-α單克隆抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。客服
簡單介紹:
中文名稱:腫瘤壞死因子-α單克隆抗體
英文名稱:Anti-TNF-alpha(1F6)
產品編號:byk-0387M
產品別名:tumor necrosis factor-α; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a ; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha.
產品規格:0.1ml、0.2ml、1ml
產品用途:科研實驗
產品價格:請報價
說明書:請添加客服直接索取
抗體來源:該指標有兩種產品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應:請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-TNF-alpha(1F6)抗體,腫瘤壞死因子-α單克隆抗體產品介紹:Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
免疫印跡法是一種通過凝膠電泳分離蛋白質并通過電轉移把蛋白質轉移到吸附膜上。一經蛋白印跡后,即可通過特異性的標記抗體檢測到相應蛋白質。
十二烷基硫酸鈉-聚丙烯酰胺凝膠電泳(SDS-PAGE)可用于檢測已被SDS化學變性的蛋白。然而,某些特定的抗體不會在一個變性的蛋白質分子上識別其抗原表位,這時需要進行不含SDS的聚丙烯酰胺凝膠電泳。
蛋白印跡轉膜可以通過濕法或半干法進行。在前者,凝膠夾在印跡膜和各種過濾裝置中間,然后把整個裝置埋進一個裝滿Tris轉移緩沖的電極槽中。在后者,夾在中間的凝膠周圍只有少量的緩沖液,然后封閉在電極板之間。雖然半干法轉膜較快,但需要很好地擬合各部分的夾層配件,而濕法轉膜很少會出現問題,可以盡量保持重復實驗結果的*性。此外,如果半干法轉膜時間過長,較小分子量的蛋白質可能會從膜上轉過。
轉膜后,需對膜進行封閉,以減少非特異性蛋白結合,然后進行用一抗孵育膜,以研究感興趣的目標蛋白。單克隆抗體通常具有更高的特異性;然而許多單克隆抗體產生于某個特定肽段而不是一個天然蛋白質,因此可能無法檢測到PAGE所分離的天然蛋白。也可使用多克隆抗體,但易形成較高的背景值。因一抗通常無標記,所以需要一個可以結合在一抗Fc段的標記的二抗,以用于zui后的檢測。通常會用酶來標記二抗。酶標記后的印跡可通過化學發光酶底物及放射自顯影技術成像。被抗體檢測和標記到的蛋白質會出現在圖像的暗區。
Anti-TNF-alpha(1F6)抗體,腫瘤壞死因子-α單克隆抗體相關產品:
CXCL14/BRAK(C-X-C motif chemokine 14) CXC趨化因子14抗體
CXCL16(CXC-chemokine ligand 16) CXC趨化因子16抗體
Secretin 分泌素抗體
Secretin receptor 分泌素受體抗體
SEL1L(Suppressor of lin-12-like Protein) SEL1L抗體
Selenoprotein W 硒蛋白W抗體
Sema3A (semaphorin 3A) 臂板蛋白3A抗體
Sema3F (semaphorin 3F) 臂板蛋白3F抗體
Sema4C (semaphorin 4C) 臂板蛋白4C抗體
SFRP1(Secreted frizzled related protein 1) 分泌型卷曲相關蛋白1抗體
SGC (soluble glycoprotein C) 可溶性糖蛋白C抗體
SGLT1 (sodium-glucose cotransporter1) 鈉-葡萄糖共轉運載體1抗體
Shank1(SH3 and multiple anleyrin repead domins protein 1) shank-1抗體
Shadow/SPRN(Shadow of prion protein precuBYKor) 新朊蛋白/朊蛋白相關蛋白/沙杜(Shadoo)蛋白抗體
SHBG (Sex Hormone Binding Globulin) 性激素結合球蛋白抗體
Shiga-like toxin IIe variant subunit A[Escherichia coli 0139] 抗大腸桿菌志賀樣毒素Ⅱ型突變體(O139菌型)抗體
Shh(Sonic hedgehog) Shh信號轉導通路膜蛋白受體抗體
SHP-1 (Src homology phosphatase-1) 造血細胞磷酸酶抗體
SHP2/PTPN11(Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2) 蛋白*磷酸酶2抗體
SIP1 (survival of motor neuron protein interacting protein 1) 運動神經元存活蛋白結合蛋白1抗體
SIRT1(sirtuin 1) 沉默調節蛋白1抗體
Skp2 (S-phase kinase-associated protein 2) 細胞S相激酶相關蛋白抗體
SLC4A4(solute carrier family 4:sodium bicarbonate cotransporter NBC1) *協同轉運蛋白4-A4抗體
Slc4a7/Nbcn1(solute carrier family 4, sodium bicarbonate cotransporter, member 7) *協同轉運蛋白4-A7抗體
Slc22A17 (solute carrier famili 22A17) 可溶性載質轉運蛋白22A17抗體