上海研盟生物有限公司*品質供應Anti-COQ10B抗體,我公司銷售國內外科研產品,如生物試劑、生化試劑,一抗二抗,各種標記抗體,Elisa酶聯免疫試劑盒,放免試劑盒,培養基,各種動物血清等產品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
中文名稱:*B抗體
英文名稱:Anti-COQ10B抗體
避免反復凍融,保質期一年。
*B抗體,Anti-COQ10B抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
各種HRP酶標記成抗體的效價測定程序如下(如:羊抗鼠IgG-HRP的效價測定):
1、抗原包板:15ug/ml 小鼠IgG-pH9.6·CB液 0.1ml/孔;
2、37°C包被60min或4°C過夜;
3、PBST洗板3次,5min/次;
4、加入倍比稀釋的HRP標記抗體 0.1ml/孔;
5、37°C 30min;
6、PBST洗板5min/次×3;
7、底物液0.1ml/孔 37°C 5-10min;
8、終止液:2N H2SO4 0.05ml/孔;
9、450nm 測定OD值;
10、陽性OD值比對照值大3倍,為判斷效價稀釋度。
*B抗體,Anti-COQ10B抗體簡單介紹
別名:Coenzyme Q10; Coenzyme Q10 B; Coenzyme Q10 homolog B; Coenzyme Q10B; CQ10B_HUMAN.
規格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產品類型:一抗
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
*B抗體背景介紹:COQ10B, Coenzyme Q10, is a 247 amino acid protein encoded by the human gene COQ10B. COQ10B is a mitochondrial protein that belongs to the COQ10 family. COQ10B is an essential biological cofactor which increases brain mitochondrial concentration and exerts neuroprotective effects. Plasma COQ10B levels decrease in patients with advanced chronic heart failure (CHF) while COQ10B levels in hyperthyroid patients are found among the lowest detected in human diseases. Likewise, COQ10B is elevated in hypothyroid subjects, also in subclinical conditions, suggesting the usefulness of this index in assessing metabolic status in thyroid disorders. It is believed that secretion of adrenal hormones is in some way related to COQ10B levels, both in augmented and reduced conditions. However, since thyroid hormones have an important role in modulating COQ10B levels and metabolism, when coexistent, thyroid deficiency seems to play a prevalent role in comparison with adrenal deficiency.
免疫熒光染色與免疫組化前期處理相同,因為內源性過氧化物酶對免疫熒光實驗沒有影響,同時雙氧水反而會干擾到熒光素發光,所以這步操作在免疫熒光中不需要做。
常用的熒光顯微鏡一般有三個通路,激發波長為350nm、488nm、555nm附近,分別對應的熒光發射光顏色為藍光、綠光、紅光。
350nm常用熒光素:DAPI(復染用),Alexa Fluor 350
488nm常用熒光素:FITC,Alexa Fluor 488
555nm常用熒光素:PE、Cy3、Rhodamine B、Alexa Fluor555
另有其他常見熒光素
594nm常用熒光素:Texas Red、Alexa Fluor594
647nm常用熒光素:Cy5、APC、Alexa Fluor647
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