上海研盟生物有限公司*品質供應Anti-SMARCD3抗體,我公司銷售國內外科研產品,如生物試劑、生化試劑,一抗二抗,各種標記抗體,Elisa酶聯免疫試劑盒,放免試劑盒,培養基,各種動物血清等產品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
中文名稱:肌動蛋白依賴染色質調控因子SMARCD3蛋白抗體
英文名稱:Anti-SMARCD3抗體
肌動蛋白依賴染色質調控因子SMARCD3蛋白抗體,Anti-SMARCD3抗體簡單介紹:
別 名:60 kDa BRG 1/Brm associated factor subunit C; 60 kDa BRG-1/Brm-associated factor subunit C; BAF60C; BRG1 associated factor 60C; BRG1-associated factor 60C; CRACD3; MGC111010; Rsc6p; Smarcd3; SMRD3_HUMAN; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily d member 3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3.
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
背景介紹:SMARCD3, is a member of the SMARCD family and contains one SWIB domain. Two isoforms, isoform 1 and isoform 2 exist due to alternative splicing events. Both isoforms are expressed in placenta, salivary gland, kidney, brain, trachea, uterus, prostate, testis, thyroid, spleen and heart, while isoform 1 is also expressed in adipose tissue and skeletal muscle. Localizing to the nucleus, SMARCD3 is a component of the ATP-dependent chromatin remodeling complex SNF/SWI and is believed to play a role in nucleosome remodeling. SMARCD3 also plays an important role in the regulation of muscle development. In mice, the silencing of the gene en-coding SMARCD3 leads to defects in heart morphogenesis. In addition, both isoforms of SMARCD3 directly interact with and function as coactivators for several transcription factors.
規格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產品類型:一抗
肌動蛋白依賴染色質調控因子SMARCD3蛋白抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復凍融,保質期一年。
肌動蛋白依賴染色質調控因子SMARCD3蛋白抗體,Anti-SMARCD3抗體實驗注意事項
1、 把聚丙烯酰胺凝膠中的蛋白質電泳轉移到硝酸纖維膜上;
a. 轉移緩沖液洗滌凝膠和硝酸纖維素膜,將硝酸纖維素膜鋪在凝膠上,用5ml移液管在凝膠上來回滾動去除所有的氣泡
b. 在凝膠/濾膜外再包一張3mm濾紙(預先用轉移緩沖液浸濕),將凝膠夾在中間,保持濕潤和沒有氣泡
c. 將此濾紙/凝膠/薄膜濾紙按照廠家建議方法放入電泳裝置中,凝膠面向陰極
d. 將上述裝置放入緩沖液槽中,并灌滿轉移緩沖液以淹沒凝膠
e. 按照廠家所示接通電源開始電泳轉移
f. 轉移結束后,取出薄膜和凝膠,棄去凝膠
2、 將薄膜漂在氨基黑中快速染色,直至分子量標準顯現時取出,記錄下標準位置;
3、 用100ml水洗滌纖維素膜,必要時可用脫色緩沖液;
4、 膜置印跡緩沖液中于37℃保溫1小時;
5、 室溫下,用PBS-Tween緩沖液洗滌薄膜
6、 用封口機將薄膜封入塑料袋中,盡可能不留空氣;
7、 袋的一角剪一緩沖液的小口,用透析袋夾緊;
8、 混合:NGS(100微升),印跡緩沖液中的抗體(2.5-5毫升),加在裝薄膜的袋中,于室溫下搖動2小時(或4℃過ye);
9、 用總體積300ml PBS-Tween緩沖液,分4次在一淺盤中洗滌薄膜,每次75ml;
10、將連接*的羊抗兔IgG(40微升溶于10毫升印跡緩沖液/100微升 NGS)加在袋內,于室溫下搖動1小時;
11、按步驟9洗滌;
12、加入抗生素蛋白-HRP(40微升溶于10毫升印跡緩沖液/100微升 NGS),于室溫下搖動;
13、Western Blot中轉移在膜上的蛋白處于變性狀態,空間結構改變,因此那些識別空間表位的抗體不能用于Western Blot檢測。這種情況可以將表達目的蛋白的細胞或細胞裂解液中的所有蛋白物素化,再用酶標記親和素進行Western Blot。實驗中取膠和膜需帶手套。
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