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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Ras(G12D)[26036][100µL]CatalogNumber:26036GeneSymbol:RAS(G12D)Description:AntiRas(G12D)MouseMonoclonalAntibodyBackground:Ras(G12D)isonetypeofRasmissensemutationsatthecodon12
Ras(G12D) [26036]
[100 µL]
| Catalog Number: 26036 |
| Gene Symbol: RAS(G12D) |
| Description: Anti Ras(G12D) Mouse Monoclonal Antibody |
| Background: Ras(G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:100-1:3000 WB: 1:200-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2a |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant Ras WT and G12D proteins. Equal amount of purified Ras WT (lane 1, cat. #10172) and G12D (lane 2, cat.# 10154) proteins were loaded and blotted with anti-Ras G12D mouse monoclonal antibody(Cat. # 26036) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat. # 21021） |
| Immunofluorescence: |
| Immunofluorescence of cells expressing RAS proteins with anti RAS(G12D) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G12D) plasmid, pCDNA3-GFP Ras(WT)plasmid,pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(G12V)plasmid,pCDNA3-GFP Ras(G12C)plasmid, pCDNA3-GFP Ras(G12R)plasmid,or pCDNA3-GFP Ras(G12A)plasmid, then fixed and stained with Anti Ras(G12D) monoclonal antibody (Cat. # 26036). |
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| Publications: |
| 1. Gene Therapy 21, 888-896 (October 2014) |
| 2. J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
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