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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Ras(G13D)[26038][100µL]CatalogNumber:26038GeneSymbol:Ras(G13D)Description:AntiRas(G13D)MouseMonoclonalAntibodyBackground:Ras(G13D)isonetypeofRasmissensemutationsatthecodon13
Ras(G13D) [26038]
[100 µL]
| Catalog Number: 26038 |
| Gene Symbol: Ras(G13D) |
| Description: Anti Ras(G13D) Mouse Monoclonal Antibody |
| Background: Ras(G13D) is one type of Ras missense mutations at the codon 13. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G13D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G13D, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:100-1:3000 WB: 1:200-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: RasG13D antibody recognizes RasG13D proteins of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant Ras WT and G13D proteins with anti Ras(G13D) monoclonal antibody. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G13D (lane 2, Cat # 10155) proteins were loaded and blotted with anti Ras(G13D) mouse monoclonal antibody (Cat # 26038) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti Ras antibody (Cat # 21021). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing RAS proteins with Anti Ras(G13D) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G13D) plasmid,pCDNA3-GFP Ras(WT)plasmid, pCDNA3-GFP Ras(G13A) plasmid,pCDNA3-GFP Ras(G13V) plasmid, pCDNA3-GFP Ras(G13C)plasmid, pCDNA3-GFP Ras(G12R)plasmid or pCDNA3-GFP Ras(G13S)plasmid, then fixed and stained with anti-Ras(G13D) monoclonal antibody (Cat. # 26038). |
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| Publications: |
| 1. Gene Therapy 21, 888-896 (October 2014) |
| 2. J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
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