Description: Anti TP53(R175H) Mouse Monoclonal Antibody
Background: TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on.
Immunogen: A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin
Species Reactivity: Anti TP53(R175H) antibody recognizes TP53(R175H) of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.
Download Datasheet:
Western blot:
Western blot analysis of recombinant TP53(R175H) and wildtype proteins. Purified His-tagged TP53(R175H) protein(Cat.#10207) and wildtype protein(Cat.#10202) were blotted with Anti TP53(R175H) monoclonal antibody(Cat.# 26072).
Immunofluorescence:
Immunofluorescence of cells expressing TP53 proteins with anti TP53(R175H) antibody. HEK293T cells were transfected with pCDNA3-GFP TP53(WT) plasmid (left column) or pCDNA3-GFP TP53(R175H) plasmid (right column), then fixed and stained with anti- TP53(R175H) monoclonal antibody (Cat. # 26072).