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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Anti IDH2(R172H) Mouse Monoclonal Antibody
IDH2(R172H) [26084]
[100 µL]
| Catalog Number: 26084 |
| Gene Symbol: IDH2 |
| Description: Anti IDH2(R172H) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: A synthetic peptide from the internal region of human IDH2 which includes the mutation of R172H. |
| Applications: ELISA, WB, IHC, IF |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 IF: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant IDH2(R172H) and wild-type IDH2 proteins. Purified his-tagged IDH2(R172H) protein (Cat. # 10201) and wild-type IDH2 protein (Cat. # 10200) were blotted with anti IDH2(R172H) monoclonal antibody (Cat. # 26084) (left panel). Proteins loading were blotted with anti-IDH2 monoclonal antibody (right panel). | |
| Immunofluorescence: |
| Immunofluorescence of cells expressing IDH2 proteins with Anti IDH2(R172H) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH2(WT) plasmid (left column) or pCDNA3-GFP IDH2(R172H) plasmid (right column), then fixed and stained with Anti IDH2(R172H) monoclonal antibody (Cat. #26084). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti IDH2(R172W) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH2(R172K) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH2(R172S) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti IDH2(R140Q) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH2(R172H) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Publications: |
| 1. Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11 |
| 2. Nature 513, 110–114 (04 September 2014) |
| 3. Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015 4. Fresia Pareja, Edaise M. da Silva, [..] Edi Brogi E 02 January 2020 |
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