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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Anti ATM(F858L) Mouse Monoclonal Antibody
ATM(F858L) [26141]
[100 µL]
| Catalog Number: 26141 |
| Gene Symbol: ATM |
| Description: Anti ATM(F858L) Mouse Monoclonal Antibody |
| Background: Ataxia telangiectasia mutated (ATM) is a serine/threonine protein kinase that is recruited and activated by DNA double-strａnd breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Activity of ATM protein is under tight control, and mutation of ATM can cause disease such as Ataxia telangiectasia (AT) and cancers. |
| Immunogen: A synthetic peptide from the internal region of ATM which includes the mutation of F858L, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes ATM(F858L), but not wild type ATM protein from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant ATM(F858L) and wildtype proteins. Purified His-tagged ATM(F858L) protein (amino acids 2400-3056, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti ATM(F858L) mouse monoclonal antibody (Cat. #26141). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing ATM(F858L) protein with anti-ATM(F858L) antibody. HEK293T cells were transfected with pCDNA3-GFP ATM(WT) plasmid (left column) or pCDNA3-GFP ATM(F858L) plasmid (right column), then fixed and stained with Anti ATM(F858L) monoclonal antibody (Cat. #26141). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Anti ATM Rabbit Polyclonal Antibody | 100 μL | ￥2600 | |
| Anti ATM(F858L) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti ATM(Q2442P) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti ATM(R3008H) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti ATM(R2443Q) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
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