Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. In mammals, there are three Rac genes: Rac1, Rac2 and Rac3. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Immunogen: Recombinant full length protein of Rac1
Species Reactivity: Rac1 antibody recognizes Rac1 from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:
Western blot:
Western blot analysis of Rac1 proteins. MEF whole-cell lysate was used for western blots. The monoclonal anti-Rac1 antibody was pre-incubated without (lane 1) or with (lane 2) the antigen protein (Rac1) to show the specificity.
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Publications:
1. Cytoskeleton Volume 71, Issue 4, pages 257–272, April 2014
2. Neutrophil Methods and Protocols Methods in Molecular Biology Volume 1124, 2014, pp 251-268
3. Molecular and Cellular Neuroscience Volume 60, May 2014, Pages 10–25
4. The Journal of Immunology April 14, 2014 1301155
5. International Journal of Cancer Volume 135, Issue 11, pages 2493–2506, 1 December 2014
6. J Biol Chem. 2014 May 30;289(22):15244-58
7. Mol Cell Biol. 2014 Nov 15;34(22):4186-99
8. PLoS One. 2014 Sep 23;9(9):e108257
9. Cancer Lett. 2015 Jan 28;356(2 Pt B):382-91
10. Physiological ReportsPublished 24 October 2014Vol. 2no. e12175DOI: 10.14814/phy2.12175
11. Anticancer Research December 2014 vol. 34 no. 12 7111-7117
12. Cancer letters. March 1, 2015Volume 358, Issue 1, Pages 76–84.