Description: Anti EGFR(N826S) Mouse Monoclonal Antibody
Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of N826S, human origin.
Species Reactivity: Recognizes EGFR(N826S), but not wild type EGFR protein from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.
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Western blot:
Western blot analysis of recombinant EGFR(N826S) and wildtype proteins. Purified His-tagged EGFR(N826S) protein (702-987) (Cat. #10221) and corresponding wild-type protein (702-987) (Cat. #10210) were blotted with anti EGFR(N826S) mouse antibody (Cat. #26116).
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with Anti EGFR(N826S) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP-EGFR(N826S) plasmid (right column), then fixed and stained with anti- EGFR(N826S) monoclonal antibody (Cat. #26116).