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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
IDH2(R172K)[26163][100µL]CatalogNumber:26163GeneSymbol:IDH2
IDH2(R172K) [26163]
[100 µL]
| Catalog Number: 26163 |
| Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| Description: Anti IDH2(R172K) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas. |
| Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: recognizes R172K mutant, but not wild-type IDH2 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant IDH2(R172K) and wildtype proteins. Purified His-tagged IDH2(R172K) (lane 2) and wildtype protein (lane 1) were blotted with Anti IDH2(R172K) monoclonal antibody (Cat. #26163). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing IDH2 proteins with Anti IDH2(R172K) antibody. HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP IDH2(WT) plasmid, pCDNA3-GFP IDH2(R172H) plasmid , pCDNA3-GFP IDH2(R172W) plasmid, pCDNA3-GFP IDH2(R172G) plasmid,pCDNA3-GFP IDH2(R172M) plasmid or pCDNA3-GFP IDH2(R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #26163) |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti IDH2(R172W) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH2(R172K) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH2(R172S) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Anti IDH2(R140Q) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti IDH2(R172H) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
| Publications: |
| 1. Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11 |
| 2. Nature 513, 110–114 (04 September 2014) |
| 3. Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015 |
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