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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Orai1-L1
Orai1-L1 [21001]
[100 µL]
| Catalog Number: 21001 |
| Gene Symbol: Orai1 |
| Description: Anti Orai1 Rabbit Polyclonal Antibody (Orai1-L1) |
| Background: Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a ~35 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene. |
| Immunogen: A peptide from the extracellular loop 1 region of human Orai1 |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000, WB: 1:1000-1:2000, IHC: 1:100-1:200 |
| Concentration: |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: IgG |
| Purity: Immunogen affinity purified |
| Storage buffer: |
| Preservative: 0.01% sodium azide |
| Constituents: 50mM NaHCO3-Glycine, 0.15M NaCl, pH7.4 |
| Species Reactivity: Orai1-L1 antibody recognizes Orai1 from human and mouse. It may also recognize Orai2 and Orai3. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of Orai1 protein. Human Orai1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot in the absence (lane 1) or presence (lane 2) of antigen peptide. | |
| Immunohistochemistry | |
| Immunohistochemistry of endogenous Orai1 in NIH3T3 cells showing the plasma membrane staining. |
| Publications: |
| 1. J Pharmacol Exp Ther. 2013 May;345(2):225-38 |
| 2. Ann Allergy Asthma Immunol. 2012 Feb;108(2):103-12 |
| 3. Am J Otolaryngol. 2011 Jul-Aug;32(4):318-28 |
| 4. Histochem Cell Biol. 2010 Jul;134(1):93-102 |
| 5. Cancer Cell. 2009 Feb 3;15(2):124-34 |
| 6. Biotech Histochem. 2014 May;89(4):273-86 |
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