Background: Heterotrimeric G proteins are critical cellular signal transducers. Gα irepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored.
Immunogen: Recombinant full length protein of Gα i1
Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Gα i antibody recognizes Gα i1, Gα i2, and Gα i3 of vertebrates
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Western blot:
Western blot analysis of Gi proteins. Gα i1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blot with the polyclonal anti Gα i antibody (cat # 21006).