Description: Anti active Arl1 Mouse Monoclonal Antibody
Background: Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal.
Immunogen: Recombinant Arl1 protein (active form)
Applications: IP, IHC, IF
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP 1:50~100 dilution for IHC, IF
Species Reactivity: Arl1 antibody recognizes active Arl1-GTP from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Immunoprecipitation/Western blot:
Arl1 Activation Assay. Purified GST-tagged Arl1 proteins (Cat. #10136) were immunoprecipitated with the anti active Arl1 monoclonal antibody (Cat. # 26924) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti Arl1 monoclonal antibody (Cat. #26056). Arl1 protein input control is shown in the bottom panel.