Description: Anti Active Gαo Mouse Monoclonal Antibody
Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαorepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
Immunogen: Recombinant full length protein of active Gαo
Applications: IP, IHC
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC
Species Reactivity: Anti-active Gαo antibody recognizes active Gαo of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Immunoprecipitation/Western blot:
Gαo activation assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti Gαopolyclonal antibody (Cat. No. 21015).