Description: Anti active Arl3 Mouse Monoclonal Antibody
Background: Arl3 (Arf-like 3) is an Arf family protein. Arl3 binds microtubules to alter specific aspects of cytokinesis. It has been proposed that retinitis pigmentosa 2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery.
Immunogen: Recombinant Arl3 protein (active form)
Applications: IP, IHC, IF
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP 1:50~100 dilution for IHC, IF
Species Reactivity: Arl3 antibody recognizes active Arl3-GTP from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Immunoprecipitation/Western blot:
Arl3 Activation Assay. Purified His-tagged Arl3 proteins (Cat. #10152) were immunoprecipitated with the anti active Arl3 monoclonal antibody (Cat. #26925) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti Arl3 monoclonal antibody (Cat. #21070). Input controls are shown in the bottom panel.