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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
ActiveGαiGTP[26901][30µL]CatalogNumber:26901GeneSymbol:GnaiDescription:AntiActiveGαiMouseMonoclonalAntibodyBackground:HeterotrimericGproteinsarecriticalcellularsignaltransducers
Active Gαi GTP [26901]
[30 µL]
| Catalog Number: 26901 |
| Gene Symbol: Gnai |
| Description: Anti Active Gαi Mouse Monoclonal Antibody |
| Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαirepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαi proteins are being explored. |
| Immunogen: Recombinant full length protein of active Gαi1 |
| Applications: IP, IHC |
| Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC |
| Concentration: 1 mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG1 |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg 2+ ａnd Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti active Gαi antibody recognizes active Gαi1, Gαi2, and Gαi3 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Immunoprecipitation/Western blot: |
transfected with wild-type Gαi1 (lanes 1 and 2) or constitutively
active Gαi1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS
(lane 3). Lysates were then incubated with an anti-active Gαi monoclonal antibody
(Cat. No.26901) (top panel). The precipitated active Gαi was immunoblotted with
an anti Gαi monoclonal antibody (Cat. No. 26003). The bottom panel shows the
Western blot with anti Gαi monoclonal antibody (Cat.No. 26003) of the cell lysates.
B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or
without (lane 1)carbachol. Cell lysates were then incubated with an anti active Gαi monoclonal antibody
(Cat. No. 26901) (top panel).The precipitated active Ga i was immunoblotted with an anti Gαi rabbit polyclonal
antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti tubulin of the cell lysates.
| Related Products | |||
| Catalog# | Name | Size | Price |
| Gαi Activation Assay Kit | 20 assays | ￥6800 | |
| Active Gαi-GTP Monoclonal Antibody | 30 μL | ￥4800 | |
| Anti Gαi Mouse Monoclonal Antibody | 100 μL | ￥2800 | |
| Anti Gαi Rabbit Polyclonal Antibody | 100 μL | ￥2600 | |
| Publications: |
| 1. Biochem J. 2013 Feb 15;450(1):209-19 |
| 2. J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61 |
| 3. Cancer Res. 2012 Jan 1;72(1):122-32 |
| 4. Endocrinology. 2012 Oct;153(10):4918-28 |
| 5. Cell Signal. 2011 Mar;23(3):550-4 |
| 6. Biochim Biophys Acta. 2011 Mar;1813(3):466-72 |
| 7. Steroids Volume 77, Issue 5, April 2012, Pages 424–432 |
| 8. J Biol Chem. 2010 Aug 27;285(35):26976-86 |
| 9. Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32 |
| 10. Mol Biol Cell. 2014 Nov 5;25(22):3654-71 |
| 11. Mol Cell Biol. 2014 Nov 15;34(22):4186-99 |
| 12. J Biol Chem. 2014 May 2;289(18):12748-58 |
GI,Gai,gi
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