Description: Anti Active Gαs GTP Mouse Monoclonal Antibody
Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαsrepresents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases.
Immunogen: Recombinant full length protein of active Gαs
Applications: IP, IHC
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC
Species Reactivity: Anti active Gαs antibody recognizes active Gαs of vertebrates
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:
Immunoprecipitation/Western blot:
Gαs activation assay. Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti active Gαs monoclonal antibody (Cat. No. 26906). Immunoblot was with an anti Gαsmonoclonal antibody (Cat. No. 26006).
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Publications:
1. Am J Physiol Endocrinol Metab. 2012 Apr 1;302(7):E747-58