Description: Anti Active Gαz Mouse Monoclonal Antibody
Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαz represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαz proteins are being explored.
Immunogen: Recombinant full length protein of active Gαz
Applications: IP, IHC
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC
Species Reactivity: Anti-active Gαz antibody recognizes active Gαz of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Immunoprecipitation/Western blot:
Gαz activation assay. Purified Gαz proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti active Gαz monoclonal antibody (Cat. No. 26908). Immunoblot was with an anti Gαz polyclonal antibody.