Description: Anti Active Rap1 Mouse Monoclonal Antibody
Background: Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins.
Immunogen: Recombinant full length protein of active Rap1.
Applications: IP, IHC
Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP 1:100 dilution for IHC
Species Reactivity: Anti-active Rap1 antibody recognizes active Rap1 of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Immunoprecipitation/Western blot:
Rap1 activation assay. Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-active Rap1 monoclonal antibody (Cat. No. 26912). Immunoblot was with an anti-Rap1 polyclonal antibody (Cat. No. 21104).