MRC-5細胞
細胞名稱:人胚肺細胞
細胞簡稱:MRC-5
產品貨號:TCH-C263
種屬來源:人
組織來源:肺
疾病特征:/
細胞形態:成纖維細胞樣
生長特性:貼壁生長
培養體系: DMEM-H+10%FBS+1%P/S
配套培養基貨號:TCH-G263
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎培養基+30%FBS+10%DMSO,液氮儲存
質量檢測:細菌、真菌、支原體檢測均為陰性
MRC-5細胞是一種常用的人類胚肺細胞,是由1966年從一個正常的男性胎兒的肺部組織獲得,具有非常良好的細胞生長和增殖特性,可廣泛應用于細胞培養、病毒研究和毒性測試等領域。
該細胞系來自14周齡男性胎兒的正常肺組織,該細胞老化前能傳代42~46個倍增時間。
參考文獻:
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