Siha細胞
細胞名稱: 人子宮頸鱗癌細胞 Siha細胞
細胞別稱:Siha; SIHA
貨號:TCH-C326
規格: 1X10^6
價格:1400元 (干冰運輸需另加200元干冰費)
種屬來源:人
組織來源:宮頸
特征:宮頸鱗癌
細胞形態:上皮細胞樣
生長特性:貼壁生長
培養體系:DMEM-H+10%FBS+1%P/S
配套培養基貨號:TCH-G326
傳代比例:1:3-1:6,每2-3天換液一次
Siha細胞傳代周期:48-72 h
培養條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎培養基+30%FBS+10%DMSO,液氮儲存
質量檢測:細菌、真菌、支原體檢測均為陰性
Siha細胞來源于一位日本的手術切除的腫瘤組織。電鏡下可以看到細胞間典型的橋粒和細胞質中大量的張力絲。1975年發現有支原體污染,而后被去除。該細胞整合有HPV16基因組,每個細胞中有1~2個拷貝。
蘇州阿爾法生物除了提供細胞系購買,細胞株購買,肺癌細胞株,ht-29細胞 ,血管內皮細胞株,肝癌細胞株,腫瘤細胞株,胃癌細胞株,hela細胞株,Siha細胞,s180細胞株,hela細胞株,CHO細胞株,間充質干細胞,還提供MEM培養基,DMEM,F12培養基,RPMI培養基等基礎培養基,干細胞培養基,培養基添加劑,間充質干細胞培養基 ,培養基,低血清培養基,胎牛血清,雞血清等細胞培養試劑以及實驗耗材,實驗室儀器等。
參考文獻:
"Annunziata C, et al. Distinct profiles of TERT promoter mutations and telomerase expression in head and neck cancer and cervical carcinoma. Int J Cancer 143(5): 1153-1161, 2018. PubMed:
Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 3029430
Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153
Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
Friedl F, et al. Studies on a new human cell line (SiHa) derived from carcinoma of uterus. I. Its establishment and morphology. Proc. Soc. Exp. Biol. Med. 135: 543-545, 1970. PubMed: 5529598
Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218
Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
Olive PL, Banath JP. Multicell spheroid response to drugs predicted with the comet assay. Cancer Res. 57: 5528-5533, 1997. PubMed: 9407963"
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