Description Application Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Biochem/physiol Actions Crude source of pectinase activity, also containing cellulase and hemicellulase activities. Other Notes View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer Unit Definition One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.