Treat in Phosphomolybdic Acid(磷鉬酸溶液) 0.2% Aqueous (1-5 minutes)
Stain in Sirius Red, 0.1% in Sat'd Picric Acid , for 90 minutes.
Wash for 2 minutes in 0.01 N Hydrochloric Acid .
Rinse in 70% Alcohol for 45 seconds.
Dehydrate, clear and mount in Permount (封片劑)
參考文獻:
Dolber, Paul C. and Madison S. Spach. Picrosirus Red Staining of Cardiac Muscle Following Phosphomolybdic Acid Treatment, Stain Technology, Vol. 62 (1987) 23 – 26.