產品名稱：Anti-CENTB1/ACAP1抗體,胞吞再循環相關銜接蛋白CENTB1抗體
產品編號：BYK-13843R
英文名稱：Anti-CENTB1/ACAP1
中文名稱：胞吞再循環相關銜接蛋白CENTB1抗體
英文別名：ACAP 1;
ACAP1;
ACAP1_HUMAN;
ANK repeat and PH domain-containing protein 1;
Arf GAP with coiled coil ANK repeat and PH domains 1;
Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1;
Arf-GAP with coiled-coil;
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1;
ArfGAP with coiled-coil, ankyrin repeat and PH domains 1;
CENT B1;
Centaurin beta 1;
Centaurin-beta-1;
CENTB 1;
Cnt b1;
Cnt-b1;
Cntb1;
KIAA0050.
產品規格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-CENTB1/ACAP1抗體,胞吞再循環相關銜接蛋白CENTB1抗體英文介紹：ACAP1 is a member of the ADP ribosylation factor family of ARF6 GTPase-activating proteins (GAP). GAPs are important regulators of Arf function by controlling the return of ARF to its inactive state. ACAP1 is related to AGAP1 and ASAP1, and all three proteins are similarly expressed in fibroblast cells such as NIH/3T3. Internalization and recycling of integrin receptors is important in cell adhesion and migration modulation, and once inside a cell, proteins and membranes are transported to the endosome where they are sorted for recycling or degradation. ACAP1 promotes cargo sorting by associating directly to recycling cargo proteins. Preventing this interaction inhibits protein recycling. ACAP1 binds transferrin receptors, promoting their transport to the plasma membrane from the endosome. Akt induced phosphorylation of ACAP1 at Ser 554 regulates ACAP1 interaction to integrin in endosomes, and downregulation of Akt or ACAP1 may inhibit cell migration on Fibronectin.
此產品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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Anti-CENTB1/ACAP1抗體,胞吞再循環相關銜接蛋白CENTB1抗體抗體溶解方法及分裝：
1、購買抗體后，查看標簽上的規格，如：0.1ml、0.2ml
2、以規格為 0.1ml 的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加 0.1ml 雙蒸水到試管內，混勻，-20℃保存，若為其它規格（如：0.2ml）的凍干粉抗體，則加與標簽上規格*的（如：0.2ml）雙蒸水溶解，
混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規格的 50%）雙蒸水到試管內，再加 0.05ml（規格的 50%）
的甘油到試管內，混勻，-20℃保存，若為其它規格（如：0.2ml）的凍干粉抗體，則加標簽上規格的 50%（如：0.1ml）雙蒸水，再加
標簽上規格的 50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
抗體溶解后，將其分裝成 5-10 支（以 0.1ml 抗體為例，20ulx5 支或 10ulx10 支）， -20℃保存，用
一支取一支，避免反復凍溶。
另：預試驗可做幾個梯度 1：100、1：200、1：400 稀釋，如果背景高，還可再稀釋，選一個zui
佳的稀釋比例，再正式做，效果。
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