產品名稱:Anti-SACM1L抗體,肌動蛋白抑制因子1抗體
產品編號:BYK-19362R
英文名稱:Anti-SACM1L
中文名稱:肌動蛋白抑制因子1抗體
英文別名:Phosphatidylinositide phosphatase SAC1;
SAC1;
SAC1 suppressor of actin mutations 1-like (yeast);
SAC1_HUMAN;
SACM1L;
suppressor of actin 1;
Suppressor of actin mutations 1-like protein.
產品規格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-SACM1L抗體,肌動蛋白抑制因子1抗體產品現貨銷售,其他同類產品:
byk-2197R 鴨甲型肝炎病毒聚蛋白抗體 Anti-duck hepatitis A virus polyprotein
byk-12197R 同源盒蛋白D13抗體 Anti-HOXD13
byk-10171R 前列腺腫瘤高表達蛋白1抗體 Anti-PTOV1
byk-0171R 登革熱病毒抗體 Anti-Dengue virus envelope glycoprotein E
byk-10399R 蛋白激酶C zeta 抗體 Anti-PKC zeta
byk-0399R DTX1抗體 Anti-Deltex 1
byk-11692R 肌側索硬化癥相關蛋白HECW1抗體 Anti-HECW1/NEDL1
byk-1692R 凋亡相關蛋白激酶3抗體 Anti-DAPK3/ZIP Kinase
byk-1713R 凋亡相關蛋白激酶2抗體 Anti-DAPK2/DRP1
byk-11713R 細胞骨架相關蛋白1/微管蛋白折疊輔助因子B抗體 Anti-CKAP1/TBCB
byk-11687R 枯草溶菌素轉化酶1抑制劑抗體 Anti-PROSAAS
byk-1687R 凋亡相關蛋白激酶1抗體 Anti-DAPK1
byk-11350R 微管驅動蛋白家族成員1A抗體 Anti-KIF1A
byk-1350R 死亡相關蛋白5抗體 Anti-DAP-5/p97
byk-11296R 羥基類固醇(17β)脫氫酶4/17β-HSD4抗體 Anti-HSD17B4
byk-1296R 防御素β2抗體 Anti-Defensin beta2
byk-0874R DEK基因結合蛋白抗體 Anti-DEK
byk-12165R 延伸突觸蛋白3抗體 Anti-FAM62C
byk-2165R 防御素β1/Defensin β1抗體 Anti-beta Defensin 1
byk-11163R 角質蛋白β抗體 Anti-SPRR3/Cornifin B
byk-11697R 舞蹈癥相關相互作用蛋白1/雌激素相關受體樣蛋白1抗體 Anti-HIPPI
byk-1697R 防御素α1/3抗體 Anti-DEF1/α Defensin 1/3
byk-0180R 多巴胺脫羧酶抗體 Anti-DOPA Decarboxylase
byk-10180R 腺相關病毒5 VP1抗體 Anti-AAV5 capsid protein VP1
byk-2599R DNA損傷結合蛋白2抗體 Anti-DDB2
byk-2588R DNA損傷結合蛋白1抗體 Anti-DDB1
byk-0993R DDX58抗體 Anti-DDX58
byk-1716R 誘捕受體3抗體 Anti-DcR3/TR6
byk-11716R cereblon蛋白抗體 Anti-CRBN
byk-2493R 腫瘤壞死因子配體超家族成員12A抗體 Anti-TNFRSF12A/TWEAKR/CD266
Anti-SACM1L抗體,肌動蛋白抑制因子1抗體轉膜(Transfer):
我們*在Western實驗中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜(二氟化樹脂膜膜孔徑:0.45um)也可以使用,但硝酸纖維素膜比較脆,在操作過程中特別是用鑷子夾取等過程中容易裂開。膜的使用請參考生產商的*使用步驟。
通常如果使用Bio-Rad的標準濕式轉膜裝置,可以設定轉膜電壓120V,轉膜時間為30-60分鐘。具體的轉膜時間要根據目的蛋白的大小而定,目的蛋白的分子量越大,需要的轉膜時間越長,目的蛋白的分子量越小,需要的轉膜時間越短。
在轉膜過程中,特別是高電流快速轉膜時,通常會有非常嚴重的發熱現象,把轉膜槽放置在冰浴中進行轉膜。轉膜的效果可以觀察所使用的預染蛋白質分子量標準,通常分子量zui大的1-2條帶較難全部轉到膜上。轉膜的效果也可以用麗春紅染色液對膜進行染色,以觀察實際的轉膜效果。也可以用考馬斯亮藍快速染色液對完成轉膜的SDS-PAGE膠進行染色,以觀察蛋白的殘留情況。
Anti-SACM1L抗體,肌動蛋白抑制因子1抗體
英文介紹:The Saccharomyces cerevisiae SAC1 gene modulates yeast actin function and alleviates the essential requirement for phosphatidylinositol transfer protein (sec14p) activity in Golgi secretory function. The SAC1 gene product (Sac1p) is an integral membrane lipid phosphatase of the endoplasmic reticulum (ER) and the Golgi complex and contains a Sac phosphatase domain (1-2). Sac1p functions in a wide range of cellular processes including inositol metabolism, actin cytoskeletal organization, endoplasmic reticulum ATP transport, phosphatidylinositol-phospha-tidylcholine transfer protein function and multiple-drug sensitivity (3). Sac1p is an important regulator of microsomal ATP transport, providing a link between inositol phospholipid signaling and ATP-dependent processes in the yeast ER (4). Defects in Sac1p relieves the requirement for Sec14p by altering phospholipid metabolism to expand the pool of diacylglycerol in the Golgi (5). Sac1p dysfunction exerts its pleiotropic effects on yeast Golgi function, the organization of the actin cytoskeleton, and the cellular requirement for inositol, through altered metabolism of inositol glycerophospholipids (6). These effects suggest the secretory and cytoskeletal activities are coordinated to achieve proper spatial regulation of secretion in S. cerevisiae (7).
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
咨詢及訂購的方法:
1.可來電向我們直接咨詢及訂購。:021-6034 0820,158 0061 4926
2.可加客服或交談,向我司在線人員咨詢及訂購。
3.可通過郵件咨詢及訂購。